EpigenTek presents the new CUT&LUNCH RNA Immunoprecipitation (RIP) Kit
Lenghty protocols? Delays? Take back your time with CUT&LUNCH RIP by EpigenTek.
CUT&LUNCH (Cleavage Under Target and Liberate Unique Nucleic Complex Homogenously), was recently developed by EpigenTek to provide a more rapid and efficient alternative to ChIP and CUT&RUN for studying in vivo DNA-protein interactions. The same innovative technology has now been incorporated into the new CUT&LUNCH RIP kit for ultra-fast, highly specific enrichment of target protein-RNA complexes.
CUT&LUNCH RIP is the quickest and most convenient way to enrich for RNA binding protein-specific RNA complexes.
Which are the advantages?
- Extremely Fast 3-Step Protocol: Start directly from intact cells without needing lysis and crosslinking. The entire procedure of the assay can be finished within 1 h and 50 min, minimizing RNA damage and loss of disassociated RNA binding components and preserving native protein-RNA structure.
- Enhanced Specificity and Minimized Background: Unique nucleic acid cleavage enzymes ensure low sequence bias by cleaving or removing RNA sequences at both ends of the target protein/RNA complex without affecting RNA occupied by the target protein. The liberated non-specific protein-RNA complexes are selectively eliminated, allowing for the recovery of only the antibody-bound complexes. As a result, target protein-enriched regions can be reliably achieved and identified through high-resolution mapping.
- Low Input Materials: Robust unbound RNA cleavage and immunocapture are all processed in the same single tube. This method uses both cells and tissues and allows for maximal degradation protection of the target protein with minimal sample loss. As a result, the input cell amount can be as few as 20,000 cells, <5% of the minimal amount required by traditional RIP.
- Wide Suitability: Ideal for cultured cells as well as fresh and frozen tissues from various species for different RNA binding proteins while maintaining both assay specificity and sensitivity.
- Highly Convenient The kit contains all required components, making the EpiNext™ CUT&LUNCH RNA Immunoprecipitation (RIP) Kit both convenient and cost-effective.
Extremely Fast 3‑Step Protocol