ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Dettagli prodotto

• Codice: E-BC-K235-M_96T
• Marca: Elabscience
• Link: Apri link
• Stoccaggio: This product can be stored at 2~8°C for 12 months with shading light.
• Detection range: 0.75-72.30 IU/L
• Metodo di rilevazione: Microplate reader
• Tipo di campione: serum;plasma;animal tissue;cells;cell culture supernatant
• Sensibilità: 0.75 IU/L
• Ig target: ALT;GPT;Glutamic-pyruvic transaminase
• Area di ricerca: Liver and Renal Function