Carboxylesterase (CES) is a polymeric protein that can catalyze the hydrolysis of carboxylate, thioester, carbamate and amide esters. CES can release effective drugs from some insoluble carboxylate prodrugs through hydrolysis reaction according to its hydrolysis characteristics. Secondly, due to its high catalytic efficiency and high specificity, CES can also regulate various metabolic functions (metabolism of ester substances, gene expression, material transport and detoxification, etc.). Therefore, detecting the activity of CES is of great significance for the early diagnosis of cancer and the research of biomedicine. CES catalyzed substrate reaction to produce chromogenic substance has maximum absorption at wavelength 405 nm, and CES enzyme activity is calculated by measuring OD value at 405 nm.
