Attendere..
LGC Biosearch Technologies introduced a new addition to their competent cell portfolio designed to deliver exceptionally high plasmid yields for plasmid production applications. It’s called Endura™ High-Yield Electrocompetent Cells (60902-1), a solution optimized for maximizing quality plasmid production while maintaining strong transformation performance.
What’s New:
- Engineered for high-yield, high-quality plasmid production
- Delivers 3–50x higher plasmid DNA yields than standard strains
- Transformation efficiency ≥1 × 10¹⁰ cfu/µg DNA
- Maintains stability of complex, repetitive constructs, including plasmids with long terminal repeats
- Select Grade (FMU) option supports scalable animal origin free (AOF) plasmid manufacturing.
Ideal Applications:
- High-yield plasmid production for synthetic biology and cloning workflows
- Propagation of complex viral vector plasmids including adenoviral and lentiviral constructs
- Plasmid DNA template production for in vitro transcription (IVT) and mRNA synthesis applications.
Higher Plasmid Yield vs Traditional Endura cells
Endura™ High‑Yield Electrocompetent Cells deliver substantially higher plasmid yields than traditional Endura cells and comparable commercial electrocompetent strains.
Figure 1. Superior plasmid yield with Endura High-Yield Electrocompetent Cells.
All electrocompetent cells were transformed with the same ~3 kb plasmid. Single colonies were cultured in 10 mL LB in individual 250 mL flasks. Plasmid DNA was extracted from 1 mL culture using standard procedures. Note: Increased plasmid yield will result when cultures are grown in well aerated culture conditions (for example, 10 mL culture in 250 mL flask vs. 96-well format).
Stable Propagation of Repetitive Sequences
Endura™ High‑Yield Cells reliably maintain plasmids containing multiple direct repeats and complex repeat structures.
a) 
b)
Figure 2. Stable Replication of Plasmids with multiple repeats in Endura™ High-Yield Cells. A plasmid with a) two 30 bp repeats separated by a short sequence and b) 6 consecutive 30 bp repeats were transformed into Endura™ High-Yield Electrocompetent Cells.
Integrity of Lentiviral-like Constructs
Endura™ High‑Yield Electrocompetent Cells reliably maintain plasmids with long terminal repeats (LTRs) with minimal concatemer formation.
Figure 3. Endura™ High-Yield Cells Maintain Integrity of Lenti-like Plasmids
A plasmid containing Lenti-viral–like sequences with 181 bp long terminal direct repeats was transformed into Endura™ High-Yield Electrocompetent Cells. All 10 tested colonies retained the correct plasmid size with minimal concatemer formation.
Do you want more information? Contact us at info@temaricerca.com