Controlled inducible expression

Expresso® Rhamnose

Fast: 5-second, directional cloning with no enzyme incubations,
ligation, or vector prep (see Expressioneering™ Technology)

Rhamnose-inducible for tunable protein expression.

Single strain for transformation and expression.

High-throughput Protein Expression.

Autoinduction reagents included.

E. coli expression

Optional SUMO Solubility Tag

Application Examples

Toxic protein expression control

Autoinduction 

Rapid expression screening

Kit content

The Expresso Rhamnose Cloning & Expression System contains pre-processed pRham N-His and/or pRham C-His Vector DNA, single-transformation E. cloni 10G Chemically Competent Cells (SOLOs), and the auto-induction reagents 20% Rhamnose solution and 15% Glucose solution. Also included are N-His and/or C-His Positive Control Insert DNAs, transformation positive control pUC DNA, and forward and reverse PCR primers to confirm clones.

The Expresso Rhamnose SUMO Cloning and Expression System contains pre-processed pRham N-His SUMO Vector DNA, single-transformation E. cloni 10G Chemically Competent Cells (SOLOs), and the auto-induction reagents 20% Rhamnose solution and 15% Glucose solution. Also included are SUMO Positive Control C Insert DNA, transformation positive control pUC DNA, SUMO Express Protease, SUMO Cleavage Control Protein, and forward and reverse PCR primers to confirm clones.

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