Solubility improvement (SUMO tag)

Expresso® SUMO

Fast: 5-second, directional cloning with no enzyme incubations, ligation, or vector prep (see Expressioneering™ Technology)

Increases expression efficiency and decreases likelihood of inclusion bodies.

Tightly-controlled expression of N-terminal 6xHis-tagged proteins with cleavable SUMO solubility tag

Recover native protein with SUMO Express Protease.

E.coli expression

Systems available with expression under the control of T7 or tunable Rhamnose promoters

Available in all E. coli kits:
Expresso Biotin, Expresso Rhamnose, Expresso T7

Application Examples

Production of native protein

Expressing insoluble targets

Kit content

The Expresso T7 SUMO Cloning and Expression System contains pre-processed pETite® N-His SUMO Vector DNA, HI-Control™ 10G Chemically Competent Cells for cloning, and HI Control BL21(DE3) Chemically Competent Cells for protein expression. Also included are SUMO Positive Control C Insert DNA, transformation positive control pUC DNA, SUMO Express Protease, SUMO Cleavage Control Protein, and forward and reverse PCR primers to confirm clones.

For more informations on cloning products, please click here