T7 routine expression

Expresso® T7

Fast: 5-second, directional cloning with no enzyme incubations, ligation,
or vector prep (see Expressioneering™ Technology)

IPTG-inducible for maximal protein expression.

Supplied with two competent HI-Control™ strains for cloning and expression.

N- or C-terminal 6X His-tagged proteins

Also see Expresso Rhamnose for routine protein expression and Expresso SUMO for
solubility improvement

E. coli expression

T7 Promoter

Application Examples

Routine protein expression

Maximum expression levels

Kit content

The Expresso T7 Cloning & Expression System contains pre-processed pETite® N-His and/or pETite C-His Vector DNA, HI-Control™ 10G Chemically Competent Cells for cloning, and HI-Control BL21(DE3) Chemically Competent Cells for protein expression. Also included are N-His and/or C-His Positive Control Insert DNAs, and transformation positive control pUC DNA.

For more informations on cloning products, please click here