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Fluorescent Microspheres

Non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol.
Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. A general coupling protocol is provided in TechNote 205, Covalent Coupling.

Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the bead matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide.

COMPELTM are highly uniform superparamagnetic microspheres ideal for applications that demand uniform particle response, such as miniaturized bioassays and separations. COMPELTM are comprised of iron oxide crystals dispersed in a polymer matrix, with a functional polymer overcoating for the encapsulation of magnetite and the introduction of reactive groups. As they are polymer-based, they retain the low density that is advantageous for biomolecular assays. Our initial offerings include 3?m, 6?m, and 8?m beads with measured CVs of ≤ 5% and high levels of COOH-functionalization

StarLightTM Calibration Slides feature vibrant ~6?m fluorescent microspheres dyed with a single fluorophore for basic imaging checks and calibration. Our four standard versions are appropriate for use with common microsphere filter sets: Glacial Blue (360, 450), Dragon Green (480, 520), Envy Green (525, 565), and Flash Red (660, 690). The calibration slides are available individually or as the full 4-slide collection


Fluorescent Microspheres

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