seqWell launches the new MosaiX™ Library Prep Kit!

Combine the workflow simplicity of tagmentation, the reduced insertion bias of TnX, & the performance benefits of ligation and you’ve got MosaiX Library Prep using seqWell’s directional tagmentation for population genomics….complexity made simple.

• TnX, next-generation transposase
• Simple 90-minute workflow (35 minutes or less hands-on)
• Produces high complexity libraries
• Uniform sequence coverage
• Low duplication rates

MosaiX 90-minute Workflow

“I’ve been really impressed with the outstanding performance of seqWell’s MosaiX library preparation kit. Directional tagging, driven by their TnX transposase,
reliably resulted in libraries with high complexity and uniform coverage that provided for more precise sequencing data.”
– Massimo Delledonne, Professor of Genetics at University of Verona, Italy

The TnX Difference
Reduced insertion site bias

Whole Exome Sequencing

Exome metrics at 6 Gb for libraries generated using MosaiX Library Prep were extremely comparable to libraries generated using enzymatic fragmentation followed by ligation. Additionally, MosaiX Library Prep outperformed bead-linked Tn5 transposition in terms of duplication rate, library complexity (HS Library Size), and % of Zero Coverage Targets – highlighting the TnX performance difference!

TnX finds those missing exome targets!

• Clinically relevant targets can be missed when using other library preparation methods such as bead-linked tagmentation using Tn5
• Reduced TnX insertion bias and higher molecular complexity of MosaiX libraries can access difficult regions of
• the genome that Tn5 transposase cannot

Whole Genome Sequencing

MosaiX libraries achieve higher coverage compared to libraries prepared with bead-linked Tn5 transposition with the same total PF Gb. Duplication rate is lower and estimated library size is higher for MosaiX, indicating a more complex whole genome library.