| Code | EK0525 |
| Brand | Boster |
| Title | Human TNF Alpha PicoKine"! ELISA Kit |
| Description | Human TNF Alpha PicoKine”! ELISA Kit |
| Size | 1 x 96T |
| alternative_name | Tumor necrosis factor |
| Scheda prodotto | www.bosterbio.com/datasheet.php?sku=EK0525 |
| general_information | Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Hu Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Hu TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. |
| protein_id | P01375 |
| range_detection | 15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma) |
| sensitivity | <1pg/ml |
| storage | Store at 4Ú C for 6 months, at -20Ú C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Detection method | ELISA |
| Sample Type | cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| Target species | Hu |
| Assay type | complete kit |
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