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CodeFEK0365
BrandBoster
TitleMouse GM-CSF PicoKine™ Fast ELISA Kit
DescriptionMouse GM-CSF PicoKine™ Fast ELISA Kit
Size1 x 96T
alternative_nameGranulocyte-macrophage colony-stimulating factor
concentrationN/A
Scheda prodottoLINK
general_informationGranulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Hu GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Hu GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa.
protein_idP01587
range_detection15.6pg/ml-1000pg/ml
sensitivity<1pg/ml
storageStore at 4Ú C for 6 months, at -20Ú C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Detection methodELISA
Sample Typecell culture supernates, serum and plasma(heparin).
Target speciesMouse
Target_igN/A
Assay typecomplete kit