Code | FEK0365 |
Brand | Boster |
Title | Mouse GM-CSF PicoKine™ Fast ELISA Kit |
Description | Mouse GM-CSF PicoKine™ Fast ELISA Kit |
Size | 1 x 96T |
alternative_name | Granulocyte-macrophage colony-stimulating factor |
concentration | N/A |
Scheda prodotto | LINK |
general_information | Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Hu GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Hu GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa. |
protein_id | P01587 |
range_detection | 15.6pg/ml-1000pg/ml |
sensitivity | <1pg/ml |
storage | Store at 4Ú C for 6 months, at -20Ú C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Detection method | ELISA |
Sample Type | cell culture supernates, serum and plasma(heparin). |
Target species | Mouse |
Target_ig | N/A |
Assay type | complete kit |