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CodeEK0527
BrandBoster
TitleMouse TNF Alpha PicoKine"! ELISA Kit
DescriptionMouse TNF Alpha PicoKine”! ELISA Kit
Size1 x 96T
alternative_nameTumor necrosis factor
Scheda prodottowww.bosterbio.com/datasheet.php?sku=EK0527
general_informationTumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Hu Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Hu TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
protein_idP06804
range_detection15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(mouseserum,plasma)
sensitivity<1pg/ml
storageStore at 4Ú C for 6 months, at -20Ú C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Detection methodELISA
Sample Typecell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Target speciesMouse
Assay typecomplete kit